EFFECT OF DIETS CONTAINING FISH PROTEIN HYDROLISATES ON GROWTH AND IMMUNE PERFORMANCE OF ASIAN SEABASS (Lates calcarifer Bloch) WHEN REARED IN FARM CONDITIONS

Romi Novriadi¹, Tinggal Hermawan¹, Muh Kadari¹, Dikrurrahman¹, Ibtisam^2, Mikael Herault³, Vincent Fournier³ dan Paul Seguin³

1) Batam Mariculture Development Center, Directorate General of Aquaculture, Ministry of Marine Affairs and Fisheries, Republic of Indonesia
2) Master Student at Human Nutrion and Rural Development, Faculty of Bioscience Engineering, University of Ghent
3) Aquativ (DIANA Division, Member of SYMRISE Group), ZA du Gohélis 56250 Elven, France
*: Corresponding author : Romi Novriadi, Jl. Raya Barelang Jembatan III, Pulau Setoko, Batam – 29422. E-mail: Romi_bbl@yahoo.co.id

A B S T R A C T

We investigated the effects of fish protein hydrolysate (FPH) on zootechnical performance and immune response of the Asian Seabass Lates calcarifer Bloch. Experimental fish were fed with 3 diets: a local commercial diet (control), coated or not, with 2 and 3% FPH (w/w). Twelve thousand Asian Seabass juveniles (5.88±0.56 g) were divided into three groups and two replicates reared in nursery tanks (2000 L). The remaining fish were then used for grow-out experiment in floating net cages (1m x 1 m x 3 m). Zootechnical performances were assessed at both stages with following indicators: total weight gain (TWG), % relative weight gain (% RWG), % specific growth rate (% SGR), final weight (g) and final length (cm). At the end of each trial period, fish immune status was assessed through blood sampling and the measurement of Neutrophile (%), Monocyte (%), Lymphocyte (%), Macrophage (10⁵ cell/mL), Leukocyte (10³ cell/mL) and Phagocytes activity (%). At the end of the nursery trial, an immersion bacterial challenge with Vibrio parahaemolyticus (10⁵ cells mL^-1) was implemented. The results showed that dietary FPH supplementation significantly influenced the growth and immune status of Asian Seabass when compared to the control group. Fish fed FPH supplemented diet yielded higher growth rates and survival rates than non supplemented group. Fish phagocytic activity and resistance to a bacterial challenge were also improved by dietary FPH supplementation. These results may be related to the significant changes observed in fish leukocyte profiles, when fed FPH supplemented diets. Altogether, these results show the positive contribution of FPH to the sustainability of Asian seabass farming.

Keywords: Functional hydrolysates, Asian Seabass, Zootechnical performance, Immune response, Phagocytic activity, Nursery, Grow out

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