MINI REVIEW

METODE DAN APLIKASI HIGH TROUGHPUT SCREENING PADA EKSPLORASI SENYAWA BIOPROSPEKTIF BIOTA LAUT SPONS DAN KARANG LUNAK

Hedi Indra Januar^1,2,), Dafit Ariyanto^1,3), Mohammad Muhaemin^1,4), Rr. Puji Hastuti Kusumawati^1,5), dan Sugeng Putranto^1,6)

1. Program Studi Ilmu Kelautan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Jl. Rasamala, Kampus IPB Darmaga, Bogor 16680.
2. Balai Besar Penelitian dan Pengembangan Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan, Jl. KS Tubun Petamburan VI Slipi Jakarta Pusat 10260.
3. Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Asahan, Jl. A Yani Kisaran, Sumatera Utara 21224.
4. Program Studi Budidaya Perairan, Fakultas Pertanian, Universitas Lampung, Jl. S. Brojonegoro No 1, Bandar Lampung 35145.
5. Fakultas Politeknik Negeri Pontianak, Jl. A Yani, Pontianak 78124
6. PT. Donggi-Senoro LNG, Sentra Senayan II Lantai 8, Jl. Asia Afrika Senayan Jakarta 10270.

E-mail:idjanuar@kkp.go.id

ABSTRACT

The marine sessile organisms, i.e. sponge and soft coral, are resources which potential to produce new bioprospecting chemicals. Within the exploration strategy trough these species, an application of high troughput screening (HTS), is one of the most important step to direct the research for gaining new chemicals with pharmacological prospective. The phylosophy of HTS methods is scaling the analysis start from the sample which has the most probable to contain new compounds and efficiency of sample used as the raw material from marine resources considerably smaller than terestrial resources. This mini review presents the HTS technique for marine organims, expecially for sponge and soft coral, which includes dereplication, NMR ‘phytochemistry’, HPLC, and spectroscopic and bioassay techniques. Application of these techniques to sponges and soft corals collected from Seribu Islands waters gained 18 known bioactive chemicals and 1 new compound from soft coral Nephthea sp.

Keywords: Bioprospecting, High Troughput Screening,antitumor, antibacterial, sponge, soft coral.

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