Penggunaan Ekstender Madu yang Dikombinasikan dengan Krioprotektan Berbeda pada Pengawetan Sperma Ikan Nilem (Indonesian Sharkminnow, Osteochilus hasseltii Valenciennes, 1842)

Ade Sunarma^₁, Dewi Wisudiyanti Budihastuti² dan Yulia Sistina³

1. Balai Besar Pengembangan Budidaya Air Tawar (BBPBAT), Sukabumi.
2. Jurusan Perikanan dan Kelautan, Fakultas Sains dan Teknik, Universitas Jenderal Soedirman, Purwokerto
3. 3Fakultas Biologi,Universitas Jenderal Soedirman, Purwokerto

Diterima 11 Maret 2010; disetujui 5 Mei 2010

ABSTRACT

Sperm cryopreservation is beneficial for aquaculture industry (e.g. maintaining genetic variability of broodstock, efficient utilizing of sperm and synchronizing of artificial reproduction) and for ex-situ conservation (gene banks of endangered species, indigenous organism and valuable strain). Nilem is an representative model for investigating basic cryobiological parameters and developing a cryopreservation procedure for commercially cultured fish and endangered species in the cyprinid. The experiment was conducted to investigate effective combination of honey as an extender with dimethyl sulfoxide (DMSO) or methanol as cryoprotectants. Milt was diluted in extender (honey 0,5%) at the ratio of 1:9 then cryoprotectant was added at 5%, 10% or 15% (v/v) concentrations. Samples were stored in 0,5 mL straws, equilibrated at temperature 4-5 oC for 20 minutes, vaporized at 3 cm above surface liquid nitrogen for 7 minutes and then plunged into liquid nitrogen, where they were stored for 2 weeks. Sperm was thawed at temperature 39-40 oC for 10-15 seconds and was used to fertilize 100-200 eggs per straw. The percentage of sperm motility of pre-freezed sperm was not significantly different in all treatment and the highest post-thawed sperm motility was the combination of honey extender and DMSO 15% (63,33%). Sperm cryopreservation using honey with DMSO 15% resulted in highest hatching rate (87,97%). The utilization of honey in combination with DMSO proved to be suitable for cryopreservation of nilem sperm, especially with regard to sperm motility and success of hatching from fertilized egg by frozen-thawed sperm.

Keyword: cryopreservation, sperm, sperm motility, hatching rate, fish

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