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PENGARUH BERBAGAI KOMPOSISI TINGKAT KONSENTRASI MEDIA PUPUK UREA TERHADAP KANDUNGAN GLUKOSA Nannochloropsis sp. SEBAGAI ALTERNATIF BAHAN BAKU BIOETANOL


Dwi Ari Subekti, Purnama Sukardi dan Tjahjo Winanto


Jurusan Perikanan dan Kelautan Fakultas Sains dan Teknik Universitas Jenderal Soedirman
Jalan dr. Suparno Komp. GOR Susilo Sudarman Karangwangkal Purwokerto.
Telp. & Fak. (0281) 642360 & 7607433
email : dwiarisubekti@gmail.com


ABSTRACT


Nannochloropsis sp. is a microalgae that colonize. Nannochloropsis sp. potential as bio-ethanol, because it has a high nutrient content to meet the needs of the manufacture of bioethanol through fermentation process. Bioethanol can be made from substances that hydrolyzed starch to glucose. Efforts to use information needed for the manufacture of bioethanol glucose content of Nannochloropsis sp. by culture for 7 days. This research was conducted in the Laboratory of the Department of Fisheries and Marine UNSOED Purwokerto. The purpose of this study to determine the highest glucose content in cultures of Nannochloropsis sp., M engetahui glucose content of Nannochloropsis sp. optimal as bioethanol feedstock at different urea concentrations, and to determine the influence of water quality (temperature, salinity, and pH) on the growth of Nannochloropsis sp. This study uses the 4 treatment (A) Urea: ZA: TSP → 1: 2: 1 to a ratio of 10 g: 20 g: 10 g; (B) Urea: ZA: TSP → 2: 2: 1 to a ratio of 20 g: 20 g: 10 g; (C) Urea: ZA: TSP → 3: 2: 1 with a ratio of 30 g: 20 g: 10 g, and (D) Urea: ZA: TSP → 4: 2: 1 to a ratio of 40 g : 20 g: 10 g. Research using completely randomized design and 4 replications. The results showed that different doses of urea concentration was significantly different (P ≤ 0.05) of the glucose content and density of Nannochloropsis sp. Highest glucose content of Nannochloropsis sp. is 29.13%. Treatment B obtain optimal glucose content in urea concentration differences. Quality of water affects the growth of Nannochloropsis sp.


Keywords : bioethanol, Nannochloropsis sp., glucose, urea.



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